Новини
Контакти
Оголошення
Лабораторії
111P Effect of IFNa-2b on migrational ability of tumor cells on early stages
of breast cancer development.
T. Herheliuk1, O. Perepelytsina1, L. Ostapchenko2, M. Sydorenko1
1Department of Biotechnical Problems of Diagnostics, Institute for problems of
Cryobiology & Cryomedicine, Kiev, Ukraine, 2Educational and Scientific Centre “Institute of Biology & Medicine”, National University of Kyiv, Kyiv, Ukraine Background: Inflammatory reactions play an important role in all stages of tumor development.
A shift to the mesenchymal phenotype causes an increase of migratory capacity of tumor cells. Epithelial-mesenchymal transition (EMT) can also be caused by local inflammation. Searching for factors that can inhibit the transition of the cell population from the epithelial to the mesenchymal phenotype is very important for antitumor therapy. Interferon alfa (IFNa-2b) can be such a factor that has a direct effect on proliferation, differentiation and migration of tumor cells. The aim of this study was to compare the effect of IFNa-2b on the expression of EMT markers and on the basic cellular processes on 2D and 3D growth models.
Methods: 2Dcell culture was cultured in standard conditions with DME Mnutrientmedium(Sigma,USA). The initial cell density was 20104 cells/cm2. Concentrations of IFNa-2b were 103, 104 and 105 M/mL. For the initial generation of spheroids toDMEM nutrient medium2%carboxymethylcellulose was added (Bio-Rad,USA). The spheroid culture was maintained for 7 days on an orbital shaker (PSU-10i, Biosan, Latvia). Every 24 h cellswere counted in suspension and adhesion fractions using the routinemethod.
Cell viability was evaluated byMTT assay. The Stemi2000 software AxioVision Red 4.7 was used for image processing. The volume of spheroids was calculated by Bjerkvig formula.
Markers were detected by applying IHC method with primarymonoclonal antibodies:Ck (clone AE1/AE3,Dako,USA), vim (CloneV9,Dako,USA), EpCAM(Sigma,USA).
Results: The change of the cell growth type caused a change of the expression of some EMTmarkers (CKs, EpCAM, Vim). IFNa-2b had a cytotoxic effect on tumor cells, and decreased the migration ability. IFNa-2b caused an increasing of Ck and EpCAMexpression by 50.5%and 47.8%, respectively, compared with control in 2D cell culture. In 3D cell culture these increases were 33%and 34%, respectively, compared with the control.
Expression of vimsignificantly showed no difference compared with the control.
Conclusions: IFNa-2b stimulated the differentiation and inhibited a migrational ability of tumor cells on early stages of breast cancer development.
Legal entity responsible for the study: Department of Biotechnical Problems of Diagnostics, Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine.
Disclosure: All authors have declared no conflicts of interest.
Anals of oncology Esmo
Розробка клітинної тест-системи для визначення ефективності та механізмів діїї протипухлинних лікарських засобів
Визначення біобезпеки та цитотаксичного впливу наночастинок золота на моношаровій та сфероїдній культурі пухлинних клітин
Дослідження впливу металовмісних та вуглецевих наноматеріалів
